β-N-Acetylhexosaminidase Obtained from Chicken Liver and Chicken Serum
نویسندگان
چکیده
منابع مشابه
Pyruvate Carboxylase from Chicken Liver
On the basis of initial velocity and product inhibition studies a nonclassical Ping Pong Bi Bi Uni Uni mechanism has been proposed for pyruvate carboxylase from chicken liver. The nonclassical feature of this mechanism is the proposal that each active site on the enzyme is composed of two separate and functionally distinct catalytic sites, i.e., a separate catalytic site exists for the reactant...
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N-Acetyl-β-D-hexosaminidase (β-HexNAc’ase) (EC 3.2.1.52) was purified from rice seeds (Oryza sativa L. var. Dongjin) using ammonium sulfate (80%) precipitation, Sephadex G150, CM-Sephadex, and DEAE-Sephadex chromatography, sequentially. The activities were separated into 7 fractions (F1F7) by CM-Sephadex chromatography. Among them, F6 was further purified to homogeneity with a 13.0% yield and 1...
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The molecular weight of chicken liver o-3-phosphoglycerate dehydrogenase, as measured by sedimentation equilibrium analysis, was found to be 165,000, consistent with the measured sedimentation coefficient of 8.13 S. Sedimentation equilibrium studies in 6 M guanidine hydrochloride and gel electrophoresis in sodium dodecyl sulfate gave molecular weights of 40,000 to 43,000, indicating that the pr...
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The present investigation was undertaken in an attempt to elucidate the relationship of the fixation of CO2 in OAA’ by OAA carboxylase to that in malate catalyzed by “malic” enzyme. It was hoped that protein separation procedures would demonstrate the identity or non-identity of the two enzyme systems and that in addition purification of the carboxylase might lead to a better understanding of i...
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The iron-storage protein ferritin was found to be associated with highly purified coated vesicles (CV) from chicken liver. Chicken liver ferritin was morphologically similar to ferritin from horse spleen and could be isolated using a specific anti-ferritin monoclonal antibody. This antibody recognized a 240 X 10(3) Mr form of chicken ferritin but not the 22 X 10(3) Mr ferritin subunit after pro...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1984
ISSN: 0002-1369
DOI: 10.1080/00021369.1984.10866124